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Cloning with pBR322 (V-U)
- Course:Gene Manipulation and Genetic Engineering (V-U)
- Discipline:Basic and Health Sciences
- Institute:Virtual University
- Instructor(s): Dr. Basharat Ali
- Level:Graduate
Gene Manipulation and Genetic Engineering (V-U)
- Adaptors (V-U)
- AFLP-PCR (V-U)
- AFLPs detection in the absence of sequence information (V-U)
- Agrobacterium and monocots (V-U)
- Agarose gel electrophoresis (V-U)
- Agrobacterium mediated transformation (V-U)
- Alkaline Phosphatase (V-U)
- Application of transgenic mice (V-U)
- Applications of Microarray-Microbial gene expression analysis (V-U)
- Applications of Microarray-Profiling in human disease (V-U)
- Applications of PCR (V-U)
- Applications of siRNA (V-U)
- Applications of phage display (V-U)
- Automated DNA sequencing (V-U)
- Autoradiography (V-U)
- Bacterial artificial chromosomes (BACs) (V-U)
- Bacterial vectors for animal transfection (V-U)
- BT an insect resistance gene source (V-U)
- Bacteriophage lambda as cloning vector (V-U)
- Callus culture (V-U)
- Basic PCR reaction (V-U)
- Cassette mutagenesis (V-U)
- cDNA libraries (V-U)
- Basic techniques (V-U)
- Cell suspension culture (V-U)
- Basic Techniques of Gene Manipulation-an overview (V-U)
- Biopharmaceuticals approved (V-U)
- Chain termination or dideoxy procedure (V-U)
- Benton and Davis's plaque lift procedure (V-U)
- Biotechnology role in food (V-U)
- Blunt end ligation via linker molecules (V-U)
- Chemical transfection techniques (V-U)
- Chromosome jumping (V-U)
- Chloroplast transformation (V-U)
- Chromosome walking (V-U)
- Clone-by-clone sequencing (V-U)
- Cloning in Archaea (V-U)
- Cloning in gram +ve bacteria B. subtilis (V-U)
- Cloning in gram +ve other than E. coli (V-U)
- Cloning in Streptomyces (V-U)
- Cloning in S. cerevisiae (V-U)
- Cloning of cDNA by Homopolymer tailing (V-U)
- Cloning of large DNA fragments (V-U)
- Cloning with pBR322 (V-U)
- Comparative genomics of bacteria (V-U)
- Cosmid vectors (V-U)
- Comparative genomics of eukaryotes (V-U)
- Comparison of different cloning vectors-summary (V-U)
- Cutting and Joining DNA molecules (V-U)
- Comparative genomics of organelles (V-U)
- Competitor RT-PCR (V-U)
- Deficiencies and advantages of YACs (V-U)
- Constructing the optimal promoter (V-U)
- Degenerate primers (V-U)
- Desirable properties of plasmid cloning vehicles (V-U)
- DNA ligase to create covalent recombinant DNA (V-U)
- Disarmed Ti vectors (V-U)
- DNA Microarray (V-U)
- DNA modifying enzymes (V-U)
- DNA polymerases (V-U)
- DNA sequence databases (V-U)
- DNA vaccines (V-U)
- DNA sequencing: Benefits and Applications (V-U)
- Drought resistance transgenic crops (V-U)
- Effect of ethidium bromide on supercoiling of DNA (V-U)
- Genes and Genome (V-U)
- Genetic modification in animals (V-U)
- Ethics in gene manipulation (V-U)
- Genetic linkage mapping (V-U)
- Genome mapping (V-U)
- Expression cloning (V-U)
- Genomic DNA libraries (V-U)
- Fluorescence in situ hybridization (FISH) (V-U)
- Genomic libraries in high-capacity vectors (V-U)
- Function of T-DNA gene transfer (V-U)
- Gene augmentation therapy (V-U)
- Happy mapping (V-U)
- Fungal resistance (V-U)
- Gene medicine (V-U)
- Herbicide resistance in plants (V-U)
- Homopolymer tailing (V-U)
- Gene therapy in cancer (V-U)
- High-throughput sequencing (V-U)
- Host cell physiology can affect the level of expression (V-U)
- Gene transfer to Fish (V-U)
- Host range of plasmids (V-U)
- Gene transfer to Xenopus (V-U)
- Host-controlled restriction modification (V-U)
- Immunochemical screening (V-U)
- Improved methods for cDNA cloning (V-U)
- Immunochemical screening of lambdagt11 (V-U)
- Improved vectors derived from pBR322 (V-U)
- In planta transformation (V-U)
- Incompatibility of plasmids (V-U)
- Interconversion of plasmid DNA (V-U)
- Introduction (V-U)
- Isolation of genomic DNA (V-U)
- Inverse PCR (V-U)
- Knock Outs & Knock Inns (V-U)
- Lambda EMBL vectors for genomic library construction (V-U)
- Major strategies for transformation (V-U)
- Leaf disc transformation (V-U)
- Map integration (V-U)
- Markers for genome mapping (V-U)
- Mass Spectrometry (V-U)
- Maxam-Gilbert method (V-U)
- Metabolic engineering in bacteria (V-U)
- Metabolic engineering in plants (V-U)
- Methods of joining DNA fragments (V-U)
- Microinjections (V-U)
- Modifications of chain terminator sequencing (V-U)
- Orthologs and paralogs (V-U)
- Modified schemes for cloning in Cosmid vectors (V-U)
- Modified lambda phages (V-U)
- Overview of cloning strategies (V-U)
- Molecular cloning (V-U)
- Packaging phage-lambda DNA in vitro (V-U)
- Multiplex PCR (V-U)
- Particle bombardment (V-U)
- Multipurpose vectors for use in yeast (V-U)
- Partitioning and segregative stability of plasmids (V-U)
- Mutagenesis (V-U)
- pBR322, a purpose-built cloning vehicle (V-U)
- Natural plasmids as cloning vehicles (V-U)
- PCR as an alternative for cDNA cloning (V-U)
- Nested PCR (V-U)
- PCR as an alternative to genomic DNA cloning (V-U)
- Nomenclature of restriction endonucleases (V-U)
- PCR principles and procedure (V-U)
- Nucleic acid blotting (V-U)
- PCR methods for site-directed mutagenesis (V-U)
- Northern blotting (V-U)
- PCR-Agricultural sciences and environment (V-U)
- Number and size of restriction fragments (V-U)
- PCR-Forensic sciences (V-U)
- Oligonucleotide Chips (V-U)
- PCR-Medicine (V-U)
- Optimizing translation initiation (V-U)
- PCR-Molecular paleontology (V-U)
- Phage Display (V-U)
- Phasmid vectors (V-U)
- Physical mapping (V-U)
- Phenotypic traits exhibited by plasmids (V-U)
- Physical transfection techniques (V-U)
- Physical versus linkage maps (V-U)
- Polymorphic DNA detection in the absence of sequence information (V-U)
- Plant transformation (V-U)
- Preparation of cDNA for cloning (V-U)
- Plant viruses as vectors (V-U)
- Primer extension: the single primer method (V-U)
- Plasmid stability (V-U)
- Primers (V-U)
- Plasmids (V-U)
- Probe design (V-U)
- Polymerase Chain Reaction (PCR) (V-U)
- Promoter system for yeast (V-U)
- Production of recombinant proteins (V-U)
- Pronuclear microinjection (V-U)
- Properties of cDNA (V-U)
- Properties of Conjugative and non-conjugative plasmids (V-U)
- Properties of different yeast vectors (V-U)
- Protein Microarrays (V-U)
- Requirement for expression in E. coli (V-U)
- Protein trafficking (V-U)
- Resistance to bacteria (V-U)
- Rhizogenes and Ri plasmids (V-U)
- Proteomics (V-U)
- RFLP (V-U)
- Runaway plasmid vectors (V-U)
- Protoplast culture (V-U)
- Screening by functional complementation (V-U)
- Protoplast transformation (V-U)
- Screening by hybridization (V-U)
- Purification of plasmid DNA (V-U)
- RAPD (V-U)
- Screening by PCR (V-U)
- Radiation hybrid (RH) mapping (V-U)
- Real-time quantitative PCR (qPCR) (V-U)
- Screening phage display libraries (V-U)
- Recombinant proteins in plants (V-U)
- Screening strategies (V-U)
- Selectable markers for animal cell gene transfer (V-U)
- Recombinant retroviruses (V-U)
- Secretion of proteins (V-U)
- Sequencing accuracy (V-U)
- Recombinant virus production (V-U)
- Sequencing Genome (V-U)
- Regenration of fertile plants (V-U)
- Shotgun sequencing (V-U)
- Replication of phage-lambda DNA in lytic and lysogenic cycles (V-U)
- Shuttle and Expression vectors (V-U)
- siRNA Technology (V-U)
- South-western and north-western blotting (V-U)
- SNPs as physical markers (V-U)
- Southern blotting (V-U)
- Spotted DNA Arrays (V-U)
- Stability of foreign proteins in E. coli (V-U)
- Stability of mRNA and codon choice (V-U)
- Structural instability (V-U)
- Steps in cloning with lambda (V-U)
- STS in physical maps (V-U)
- T-DNA tansfer (V-U)
- Strategies to transform animal cells (V-U)
- Summary of restriction endonucleases (V-U)
- Target sites of restriction endonucleases (V-U)
- The effect of plasmid copy number (V-U)
- The flow of genetic information (V-U)
- The use of RFLPs in physical maps (V-U)
- Transfection with liposomes and lipoplexes (V-U)
- Transfection ES cells (V-U)
- Transfection with polyplexes (V-U)
- Transformation of E. coli , Electroporation (V-U)
- Transformation of fungi with exogenous DNA (V-U)
- Transformation with other organisms (V-U)
- Transgenic crops for poor quality soil (V-U)
- Transgenic animals and plants (V-U)
- Transgenic mice production (V-U)
- Transgenic model animals (V-U)
- Two plasmid strategy (V-U)
- Two-dimensional (2-D) electrophoresis (V-U)
- Types of PCR (V-U)
- Viral vectors for transfection (V-U)
- Types of restriction and modification (R-M) system (V-U)
- Ways to exploit recombinant DNA (V-U)
- Use of pSC101 for cloning (V-U)
- Western blotting (V-U)
- Yeast artificial chromosomes (YACs) (V-U)
- Vectors for DNA sequencing: bacteriophage M13 (V-U)
- What is genetic engineering? (V-U)
- Vectors for use in S. cerevisiae (V-U)